Journal: BMC Neuroscience

Article Title: The effect of an intracerebroventricular injection of metformin or AICAR on the plasma concentrations of melatonin in the ewe: potential involvement of AMPK?

doi: 10.1186/1471-2202-12-76

Figure Lengend Snippet: Western-blotting quantification of the phosphorylation rate of AMPK and ACC after i.c.v. injection of AICAR . (A) The ratio of phosphorylated AMPK at Thr172 to total AMPK following an i.c.v. injection of a synthetic CSF vehicle or AICAR. (B) The ratio of phosphorylated ACC at Ser79 to total ACC following an i.c.v. injection of a synthetic CSF vehicle or AICAR.

Article Snippet: Rabbit polyclonal antibodies to phospho-AMPKα Thr172, AMPKα1/2, AMPKβ1/β2, AMPKγ2, phospho-acetyl-CoA carboxylase (ACC) Ser79 and ACC were purchased from New England Biolabs Inc. (Beverly, MA, USA).

Techniques: Western Blot, Injection